牛源金黄色葡萄球菌α溶血素单链抗体的保护作用研究
投稿时间:2019-06-25  修订日期:2019-09-23  点此下载全文
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作者单位E-mail
张波 上海交通大学农业与生物学院 zhangbo-zinnia@sjtu.edu.cn 
王曼 上海交通大学农业与生物学院  
王凤青 上海交通大学农业与生物学院  
张蕾 上海交通大学农业与生物学院  
管玉 上海交通大学农业与生物学院  
朱建国 上海交通大学农业与生物学院  
中文摘要:为探究治疗奶牛乳腺炎的抗生素替代性生物制剂,本研究将前期获得的特异性针对金黄色葡萄球菌毒力因子α溶血素的单链抗体scFvZZ107,在小鼠感染金黄色葡萄球菌乳腺炎模型中验证其保护作用。结果显示,三种不同剂量(5、10、15μg)单链抗体处理组乳腺组织内细菌数量分别为25.42±1.3、3.33±0.53、4.8±0.78(lgcfu?mg-1),IL-1α的含量分别为49±7.91、31.37±2.26、57.34±4.48 (pg/mL),IL-1β含量分别为40.91±7.39、47.81±4.78、39.2±2.28(pg/mL),IL-8的含量分别为48.64±1.86、41.65±3.07、45.74±1.56(pg/mL),与生理盐水处理组相比,单链抗体中、高剂量处理组乳腺内金黄色葡萄球菌检出量显著减少(P<0.05),且IL-1α、IL-1β与IL-8的含量均显著降低(P<0.05)。通过观察小鼠乳腺组织HE和TUNEL染色情况,与生理盐水处理组相比,scFv中、高剂量处理组乳腺组织加完整,凋亡细胞的数量也更少,表明scFvZZ107对小鼠乳腺具有良好的保护作用。
中文关键词:金黄色葡萄球菌  α溶血素  单链抗体  小鼠乳腺
 
Preliminary study on the protective function of bovine single-chain antibody that bind α hemolysin of Staphylococcus aureus
Abstract:In order to explore antibiotic-alternative biological agents for the treatment of mastitis in dairy cows, in this study, a previously-obtained single-chain antibody scFvZZ107 specific to S. aureus virulence factor alpha hemolysin was administered in a mouse model of S. aureus infection mastitis to confirm its protection. As a result, the numbers of bacteria in the mammary glands of the three treatment groups with 5, 10 and 15μg of scFvZZ107 were 25.42±1.3, 3.33±0.53 and 4.8±0.78 (lgcfu?mg-1), respectively. The contents of IL-1α were 49±7.91 and 31.37±2.26 and 57.34±4.48 (pg/mL), respectively. The contents of IL-1β were 40.91±7.39, 47.81±4.78 and 39.2±2.28 (pg/mL), respectively. The contents of IL-8 were 48.64±1.86, 41.65±3.07 and 45.74±1.56 (pg /mL), respectively. In comparison with the saline-treated group, the numbers of S. aureus in the mammary glands treated by moderate or high doses of scFv were significantly decreased (P<0.05), and the contents of IL-1α, IL-1β and IL-8 were lower (p<0.05). The HE and TUNEL staining revealed that the proportions of intact mammary gland tissues were higher and the numbers of apoptotic cells were lower than that of the saline-treated group. Collectively, our present findings indicated that scFvZZ107 had a protective effect on mouse mastitis.
keywords:Staphylococcus aureus  alpha hemolysin  single chain antibody (scFv)  Mouse mastitis
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